Whole-genome characterization of Shewanella algae strain SYT3 isolated from seawater reveals insight into hemolysis

Future Microbiol. 2018 Dec:13:1709-1717. doi: 10.2217/fmb-2018-0267. Epub 2018 Nov 27.

Abstract

Aim: To describe the genomic characteristics of seawater-borne hemolytic Shewanella algae and its resistance genes.

Materials & methods: Whole genome sequence of S. algae SYT3 was determined using llumina MiSeq platform. Multiple-database-based analysis was performed to identify the genetic background of its hemolytic activity and the antibiotic resistance genes.

Results: S. algae SYT3 possesses a homolog of the hly operon involved in the synthesis of hemolysin. We also identified candidate genes associated with resistance to β-lactam antibiotics (bla OXA-55) and fluoroquinolone (qnrA3).

Conclusion: The study provides an insight into the hemolytic activity of S. algae. Our findings also suggested S. algae as a potential reservoir of antimicrobial resistance determinants.

Keywords: beta-lactamase OXA-55; hemolysin; hemolytic activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics
  • Bacterial Typing Techniques
  • Chromosome Mapping
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial / genetics*
  • Genome, Bacterial*
  • Hemolysin Proteins / genetics
  • Hemolysin Proteins / metabolism
  • Hemolysis*
  • Humans
  • Microbial Sensitivity Tests
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Shewanella / classification
  • Shewanella / drug effects
  • Shewanella / genetics*
  • Shewanella / isolation & purification*
  • Taiwan
  • Whole Genome Sequencing
  • beta-Lactamases / genetics

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • DNA, Bacterial
  • Hemolysin Proteins
  • RNA, Ribosomal, 16S
  • beta-Lactamases