Bone Regeneration of Canine Peri-implant Defects Using Cell Sheets of Adipose-Derived Mesenchymal Stem Cells and Platelet-Rich Fibrin Membranes

Lidan Ding; Shijun Tang; Panpan Liang; Chao Wang; Peng-Fei Zhou; Leilei Zheng

doi:10.1016/j.joms.2018.10.018

Bone Regeneration of Canine Peri-implant Defects Using Cell Sheets of Adipose-Derived Mesenchymal Stem Cells and Platelet-Rich Fibrin Membranes

J Oral Maxillofac Surg. 2019 Mar;77(3):499-514. doi: 10.1016/j.joms.2018.10.018. Epub 2018 Nov 1.

Authors

Lidan Ding¹, Shijun Tang², Panpan Liang³, Chao Wang⁴, Peng-Fei Zhou³, Leilei Zheng⁵

Affiliations

¹ Researcher, The Affiliated Stomatology Hospital, Chongqing Medical University, Chongqing; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing; Mianyang Hospital of T.C.M., Miangyang, Sichuan, China.
² Researcher, The Affiliated Stomatology Hospital, Chongqing Medical University, Chongqing; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China.
³ Laboratory Technician, The Affiliated Stomatology Hospital, Chongqing Medical University, Chongqing; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China.
⁴ Professor, The Affiliated Stomatology Hospital, Chongqing Medical University, Chongqing; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China.
⁵ Professor and Chief of Orthodontic Department, The Affiliated Stomatology Hospital, Chongqing Medical University, Chongqing; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China. Electronic address: zhengleileicqmu@hospital.cqmu.edu.cn.

PMID: 30476490
DOI: 10.1016/j.joms.2018.10.018

Abstract

Purpose: Insufficient bone volume compromises the success rate and osseointegration of immediate implantation. The objective of the present study was to engineer bone tissue by using adipose-derived stem cell (ASC) sheets and autologous platelet-rich fibrin (PRF) to enhance new bone formation and osseointegration around dental implants.

Material and methods: The proliferation and osteogenic potential of ASCs treated with autologous PRF were evaluated with CCK-8 assays, alkaline phosphatase staining, and real-time quantitative polymerase chain reaction. A 3-wall bone defect around each immediate implant was generated in the mandible and randomly treated with ASC sheets plus PRF (group A), ASC sheets only (group B), PRF only (group C), or no treatment (group D). Micro-computed tomography, biomechanical tests, fluorescent bone labeling, and histologic assessments were performed to evaluate bone regeneration capacity.

Results: The proliferation and osteogenic potential of canine ASCs were markedly enhanced by PRF. Group A exhibited considerably more new bone formation and re-osseointegration (41.17 ± 1.44 and 55.06 ± 0.06%, respectively) than did the other 3 groups. Fluorescent labeling showed that the most rapid bone remodeling activity occurred in group A (P < .05).

Conclusion: These results suggest that sheets of ASC combined with autologous PRF could be a promising tissue-engineering strategy for bone formation in immediate implantation.

MeSH terms

Animals
Blood Transfusion, Autologous
Bone Regeneration
Dental Implantation, Endosseous*
Dental Implants*
Dogs
Mesenchymal Stem Cells*
Osteogenesis
Platelet-Rich Fibrin*
X-Ray Microtomography

Substances

Dental Implants