Grass species can be classified into different functional types based on their growth strategies, and contrasting persistence strategies are observed in different grass species. Excluding seedling recruitments, changes in populations of grasses are basically a trade-off between natality and mortality of tillers. We hypothesised that the persistence pathway of perennial grasses is linked to their growth strategy, regardless whether they are growing as monoculture or as a mixture. Species with contrasting growth strategies (Arrhenatherum elatius L., Dactylis glomerata L., and Festuca arundinacea Schreb.) were cultivated as monocultures and as a mixture and their tiller natality and mortality were evaluated for two years after swards establishment. All pastures maintained their population size during the experimental period, although decreases in tiller densities occurred during the warmer season. Arrhenatherum elatius had the highest tiller mortality and natality whereas the F. arundinacea had the lowest ones. Arrhenatherum elatius had many tillers appearing in all seasons but their tillers were short-lived. Conversely, F. arundinacea and D. glomerata developed numerous tillers during autumn and winter and their tillers survived, on average, almost six and three times longer than those of A. elatius, respectively. There were no differences in tillering dynamics among populations grown in monocultures or in the mixture. Regardless of whether they were cultivated in monocultures or as a mixture, the persistence pathway of perennial grasses is linked with their growth strategies with exploitative species presenting a high tiller turnover throughout the year whereas the persistence of more conservative species is based on a high tiller survival.