Senescent cells secrete diverse array of proteins. One group of proteins, damage-associated molecular pattern (DAMP) proteins exhibit relocalization from inside to outside the cell. High Mobility Group Box 1 protein (HMGB1) is the founding DAMP member. HMGB1 relocalization from the nucleus provides a molecular signature during senescence. We provide distinct molecular techniques (immunofluorescence, immunohistochemistry, and Western blot assays) to assess HMGB1 relocalization during the initial stages of senescence.
Keywords: Alarmin; Damage-associated molecular pattern (DAMP); High Mobility Group Box 1 protein (HMGB1); Immunofluorescence; Immunohistochemistry; Protein precipitation; Western blot.