Protist Biodiversity and Biogeography in Lakes From Four Brazilian River-Floodplain Systems

J Eukaryot Microbiol. 2019 Jul;66(4):592-599. doi: 10.1111/jeu.12703. Epub 2018 Dec 21.

Abstract

The biodiversity and biogeography of protists inhabiting many ecosystems have been intensely studied using different sequencing approaches, but tropical ecosystems are relatively under-studied. Here, we sampled planktonic waters from 32 lakes associated with four different river-floodplains systems in Brazil, and sequenced the DNA using a metabarcoding approach with general eukaryotic primers. The lakes were dominated by the largely free-living Discoba (mostly the Euglenida), Ciliophora, and Ochrophyta. There was low community similarity between lakes even within the same river-floodplain. The protists inhabiting these floodplain systems comprise part of the large and relatively undiscovered diversity in the tropics.

Keywords: Ciliophora; Discoba; Euglenida; Neotropical; Ochrophyta; high-throughput sequencing; metabarcoding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodiversity
  • Brazil
  • Ciliophora / isolation & purification*
  • Euglenida / isolation & purification*
  • Lakes* / microbiology
  • Lakes* / parasitology
  • Microbiota*
  • Stramenopiles / isolation & purification*