Himalrandia tetrasperma and Wendlandia exserta medicinal plants belong to family Rubiaceae commonly known as coffee family were investigated by quantitative analysis of major bioactive compounds and analgesic effect. The analgesic potential was accessed using different parts of H. tetrasperma and W. exserta by induced acetic acid writhing and hot plate test method. Methanolic extracts of these two plants satisfactorily possesses analgesic activity. All the extracts showed good results as compared to standard drug, i.e. aspirin. Seeds of H. tetrasperma possess maximum, i.e. 86.73% inhibition at first phase where as aspirin possesses 52.73%. In second phase, leaves possess 99.8 % inhibition respectively. Hot plate analgesic activity of bark extract displayed maximum activity at 4.5h stage, i.e. 8.6±0.40. W. exserta methanolic extract of bark possesses 97.3% inhibition respectively in first phase and 99.8% in second phase. Bark extract displayed maximum activity at 4.5h stage, i.e., 9.7±0.5. Quantitative analysis of bark of H. tetrasperma possesses highest value of saponins, i.e. 30.21±0.8 where as flavonoids 17.50±1.2, phenolic compounds 23.25±0.5 and tannins 12.32±0.4. The leaf extracts of H. tetrasperma contains maximum value of phenols, i.e. 15.10±0.7 where as W. exserta bark possesses significant value of alkaloids, i.e. 16.41±0.4 and leaf extracts possesses flavonoids, i.e. 14.51±0.3, saponins 12.22±0.1 and phenolic compounds 11.31±0.4. The seeds of both plants possess significant value of tannins, i.e. 07.60±0.3.