Sulfur-based carriers were examined to enhance the nitrogen removal efficiency in a mixed anoxic⁻anaerobic-membrane bioreactor system, in which sulfur from the carrier acts as an electron donor for the conversion of nitrate to nitrogen gas through the autotrophic denitrification process. A total nitrogen removal efficiency of 63% was observed in the system with carriers, which showed an increase in the removal efficiency of around 20%, compared to the system without carriers. The results also indicated that the carriers had no adverse effect on biological treatment for the organic matter and total phosphorus. The removal efficiencies for chemical oxygen demand (COD) and total phosphorus (TP) were 98% and 37% in both systems, respectively. The generation of sulfate ions was a major disadvantage of using sulfur-based carriers, and resulted in pH drop. The ratio of sulfate in the effluent to nitrate removed in the system ranged from 0.86 to 1.97 mgSO₄2-/mgNO₃--N, which was lower than the theoretical value and could be regarded as due to the occurrence of simultaneous heterotrophic and autotrophic denitrification.
Keywords: autotrophic denitrification; elemental sulfur; heterotrophic denitrification; membrane bioreactor.