The effect of intake of sausages fortified with β-CD-I2 complex on iodine status and thyroid function: A preliminary study

Abstract

The present study evaluated influence of boiled sausages consumption fortified with β-CD-I₂ on the urinary iodine excretion (UIE) level of volunteers. Median urinary UIE level was increased from 58.02 (24.0-175.4) μg/L to 110.6 (20.5-231.6) μg/L during 10 days. Thyroid stimulating hormone (TSH) and free thyroxine (FT4) levels were determined by radio immunoassay analysis. As it was expected, intake of sausages fortified with β-CD-I₂ resulted in rise of FT4 level from 1.1 (0.95-1.25) to 1.23 (1.07-1.63) ng/dL, whereas TSH level decreased from 1.53 (0.47-3.37) mIU/L to 1.1 (0.51-3.17) mIU/L. A dynamic gastrointestinal model in vitro was used in order to determine possibility of 3,5-diiodotyrosine (DIT) formation during consumption of the fortified sausages. The DIT concentration was determined by HPLC-MS method and was found to be 0.38 ng/mL in sausage dialyzate. These findings indicate that β-CD-I₂ introduction as an iodine carrier in boiled sausages may help to improve iodine status and to control organic iodine species concentration.

Keywords: 3,5-diiodotyrosine; Boiled sausages; Iodine; Urinary iodine excretion; β-cyclodextrin.