Microarrays for High-Throughput Gene Expression Analysis of Barley

Jenny A Morris; Pete E Hedley

doi:10.1007/978-1-4939-8944-7_12

Microarrays for High-Throughput Gene Expression Analysis of Barley

Methods Mol Biol. 2019:1900:181-194. doi: 10.1007/978-1-4939-8944-7_12.

Authors

Jenny A Morris¹, Pete E Hedley²

Affiliations

¹ Genome Technology, Cell and Molecular Sciences, James Hutton Institute, Dundee, UK.
² Genome Technology, Cell and Molecular Sciences, James Hutton Institute, Dundee, UK. pete.hedley@hutton.ac.uk.

PMID: 30460566
DOI: 10.1007/978-1-4939-8944-7_12

Abstract

Microarrays remain an extremely robust and cost-efficient technology for transcriptome analysis. A custom Agilent microarray has been designed from the predicted gene transcripts of the barley genome reference. Here, we describe total RNA extraction, fluorescent labeling, hybridization, and image analysis of barley arrays. Quality control measures are detailed, along with recommendations for downstream data analysis.

Keywords: Agilent; Barley; Gene expression; Microarrays; RNA; Transcriptomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Data Analysis
Fluorescent Dyes / metabolism
Gene Expression Regulation, Plant*
Hordeum / genetics*
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis / methods*
Quality Control
RNA, Plant / isolation & purification
Staining and Labeling

Substances

Fluorescent Dyes
RNA, Plant