Neuroprotective effects of triterpenoid saponins from Medicago sativa L. against H2O2-induced oxidative stress in SH-SY5Y cells

Xue-Gui Liu; Yu-Qiu Sun; Jun Bian; Ting Han; Dan-Dan Yue; Dan-Qi Li; Pin-Yi Gao

doi:10.1016/j.bioorg.2018.11.008

Neuroprotective effects of triterpenoid saponins from Medicago sativa L. against H₂O₂-induced oxidative stress in SH-SY5Y cells

Bioorg Chem. 2019 Mar:83:468-476. doi: 10.1016/j.bioorg.2018.11.008. Epub 2018 Nov 13.

Authors

Xue-Gui Liu¹, Yu-Qiu Sun², Jun Bian², Ting Han², Dan-Dan Yue², Dan-Qi Li³, Pin-Yi Gao⁴

Affiliations

¹ College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang, Liaoning 110142, People's Republic of China; Institute of Functional Molecules, Shenyang University of Chemical Technology, Shenyang, Liaoning 110142, People's Republic of China.
² College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang, Liaoning 110142, People's Republic of China.
³ College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang, Liaoning 110142, People's Republic of China; Institute of Functional Molecules, Shenyang University of Chemical Technology, Shenyang, Liaoning 110142, People's Republic of China. Electronic address: lidanqi@yeah.net.
⁴ College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang, Liaoning 110142, People's Republic of China; Institute of Functional Molecules, Shenyang University of Chemical Technology, Shenyang, Liaoning 110142, People's Republic of China. Electronic address: gpy1981@126.com.

PMID: 30448725
DOI: 10.1016/j.bioorg.2018.11.008

Abstract

Medicago sativa L. is a forage legume plant widely distributed in all continents. Six new triterpenoid saponins, Medicagosides A-F (1-6) and five known ones (7-11) were isolated from M. sativa. Their structures were determined via HRESIMS, 1D and 2D NMR analysis. Biologically, all the isolates displayed neuroprotective activities against H₂O₂-induced damage in SH-SY5Y cells. Among them, compounds 1, 3-5 and 10 exhibited striking neuroprotective activities at 100 μM, restoring cell viability range from 79.66% to 89.03%, relative to 79.46% (100 μM) of Trolox used as the positive control.

Keywords: Medicago sativa L.; Neuroprotective effects; SH-SY5Y cells; Triterpenoid saponins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Cell Survival / drug effects
Chromans / pharmacology
Humans
Hydrogen Peroxide / pharmacology
Medicago sativa / chemistry*
Neuroprotective Agents / chemistry
Neuroprotective Agents / isolation & purification
Neuroprotective Agents / pharmacology*
Oxidative Stress / drug effects
Saponins / chemistry
Saponins / isolation & purification
Saponins / pharmacology*
Triterpenes / chemistry
Triterpenes / isolation & purification
Triterpenes / pharmacology*

Substances

Chromans
Neuroprotective Agents
Saponins
Triterpenes
Hydrogen Peroxide
6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid