Genomic features of a multidrug-resistant Enterobacter cloacae ST279 producing CTX-M-15 and AAC(6')-Ib-cr isolated from fatal infectious stomatitis in a crossed pit viper (Bothrops alternatus)

Marcelo P N de Carvalho; Quézia Moura; Miriam R Fernandes; Fábio P Sellera; Ana Helena Pagotto; Daniel R Stuginski; Rafael A Castro; Sávio S Sant'Anna; Kathleen F Grego; Nilton Lincopan

doi:10.1016/j.jgar.2018.11.009

Genomic features of a multidrug-resistant Enterobacter cloacae ST279 producing CTX-M-15 and AAC(6')-Ib-cr isolated from fatal infectious stomatitis in a crossed pit viper (Bothrops alternatus)

J Glob Antimicrob Resist. 2018 Dec:15:290-291. doi: 10.1016/j.jgar.2018.11.009. Epub 2018 Nov 15.

Affiliations

¹ Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
² Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
³ Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
⁴ Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
⁵ Laboratory of Herpetology, Butantan Institute, São Paulo, Brazil.
⁶ Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: lincopan@usp.br.

PMID: 30448520
DOI: 10.1016/j.jgar.2018.11.009

Abstract

Objectives: The widespread dissemination of extended-spectrum β-lactamase-producing Enterobacteriaceae has become a major issue in veterinary medicine. However, until now, there has been no report of bacteria with such a phenotype in infected snakes. The aim of this study was to report the first draft genome sequence of an Enterobacter cloacae isolate (SERP1) recovered from a snake with infectious stomatitis.

Methods: The whole genome of E. cloacae strain SERP1 was sequenced on an Illumina NextSeq platform and was de novo assembled using CLC NGS Cell v.10. Data analysis was performed using online tools from the Center of Genomic Epidemiology.

Results: The genome size was calculated at 4966856bp, containing a total of 4796 protein-coding sequences. The strain was assigned to sequence type 279 (ST279) and, besides the clinically relevant bla_CTX-M-15 and aac(6')-Ib-cr genes, it also presented resistance genes to β-lactams, aminoglycosides, phenicols, sulphonamides, tetracyclines, trimethoprim, quinolones and fosfomycin.

Conclusion: These data offer novel information regarding multidrug-resistant E. cloacae dissemination in wild animals and might contribute to further comparative genomic analysis.

Keywords: ESBL; Enterobacter cloacae; Extended-spectrum β-lactamase; Infectious stomatitis; Snake; Whole-genome sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Base Sequence
Bothrops / microbiology
Drug Resistance, Multiple, Bacterial*
Enterobacter cloacae / classification
Enterobacter cloacae / drug effects
Enterobacter cloacae / genetics
Enterobacter cloacae / isolation & purification*
Enterobacteriaceae Infections / microbiology
Enterobacteriaceae Infections / mortality
Enterobacteriaceae Infections / veterinary*
Genome Size
Genome, Bacterial*
Genomics
Microbial Sensitivity Tests
Stomatitis / microbiology
Stomatitis / mortality
Stomatitis / veterinary*
beta-Lactamases / genetics
beta-Lactamases / metabolism*

Substances

Bacterial Proteins
beta-Lactamases