Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy

Youhua Chen; Wenjie Liu; Zhimin Zhang; Cheng Zheng; Yujia Huang; Ruizhi Cao; Dazhao Zhu; Liang Xu; Meng Zhang; Yu-Hui Zhang; Jiannan Fan; Luhong Jin; Yingke Xu; Cuifang Kuang; Xu Liu

doi:10.1038/s41467-018-07244-4

Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy

Nat Commun. 2018 Nov 16;9(1):4818. doi: 10.1038/s41467-018-07244-4.

Authors

Youhua Chen^{1

2}, Wenjie Liu¹, Zhimin Zhang¹, Cheng Zheng¹, Yujia Huang¹, Ruizhi Cao¹, Dazhao Zhu¹, Liang Xu¹, Meng Zhang³, Yu-Hui Zhang³, Jiannan Fan⁴, Luhong Jin⁴, Yingke Xu⁴, Cuifang Kuang^{5

6}, Xu Liu^{7

8}

Affiliations

¹ State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China.
² Shanxi Provincial Key Laboratory for Biomedical Imaging and Big Data, North University of China, Taiyuan, Shanxi, 030051, China.
³ Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
⁴ Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
⁵ State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China. cfkuang@zju.edu.cn.
⁶ Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, Shanxi, 030006, China. cfkuang@zju.edu.cn.
⁷ State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China. liuxu@zju.edu.cn.
⁸ Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, Shanxi, 030006, China. liuxu@zju.edu.cn.

Abstract

Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes of live cells remains a challenging problem. In this paper, we propose a multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination microscopy with multi-angle evanescent light illumination. We demonstrate that our approach of multi-angle interference microscopy is perfectly adapted to studying subcellular dynamics of mitochondria and microtubule architectures during cell migration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Cell Movement
Cell Size
Color
Fluorescent Dyes / chemistry
Heterocyclic Compounds, 4 or More Rings / chemistry
Humans
Image Processing, Computer-Assisted
Imaging, Three-Dimensional / methods*
Microscopy, Fluorescence / methods*
Microscopy, Interference / methods*
Microtubules / ultrastructure*
Mitochondria / ultrastructure*
Osteoblasts / ultrastructure*
Staining and Labeling / methods

Substances

Fluorescent Dyes
Heterocyclic Compounds, 4 or More Rings