The presence and distribution of G protein-coupled receptor 35 (GPR35) in the human cornea - Evidences from in silico gene expression analysis and immunodetection

Exp Eye Res. 2019 Feb:179:188-192. doi: 10.1016/j.exer.2018.11.011. Epub 2018 Nov 13.

Abstract

We provide the evidence for G protein-coupled receptor 35 (GPR35) presence and distribution in the human cornea. The initial data on GPR35 gene expression were retrieved from microarray repositories and were further confirmed by western blotting and immunohistochemical analysis. Immunoblotting suggested that GPR35 exists predominantly as a dimer in corneal tissue. Moreover, corneal tissues were significantly richer in GPR35 compared to the adjacent sclera. Immunoreactivity for GPR35 was detected in normal corneas, keratoconus and Fuchs' dystrophy, mainly in the corneal epithelium and endothelium. In corneas with Fuchs' dystrophy, less intensive immunoreactivity for GPR35 in endothelium was revealed. The physiological relevance of this phenomenon requires further investigation.

Keywords: Eye inflammation; Fuchs' endothelial corneal dystrophy; G protein-coupled receptor 35; Gene expression profiling; Immunohistochemistry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Blotting, Western
  • Cornea / metabolism*
  • Endothelium, Corneal / metabolism
  • Epithelium, Corneal / metabolism
  • Female
  • Fuchs' Endothelial Dystrophy / metabolism*
  • Gene Expression Profiling
  • Gene Expression Regulation / physiology*
  • Humans
  • Keratoconus / metabolism*
  • Male
  • Microarray Analysis
  • Middle Aged
  • Receptors, G-Protein-Coupled / genetics*
  • Receptors, G-Protein-Coupled / metabolism
  • Sclera / metabolism

Substances

  • GPR35 protein, human
  • Receptors, G-Protein-Coupled