Metabolomic insights into the mechanisms underlying tolerance to salinity in different halophytes

Abstract

Salinity is among the most detrimental and diffuse environmental stresses. Halophytes are plants that developed the ability to complete their life cycle under high salinity. In this work, a mass spectrometric metabolomic approach was applied to comparatively investigate the secondary metabolism processes involved in tolerance to salinity in three halophytes, namely S. brachiata, S. maritima and S. portulacastrum. Regarding osmolytes, the level of proline was increased with NaCl concentration in S. portulacastrum and roots of S. maritima, whereas glycine betaine and polyols were accumulated in S. maritima and S. brachiata. Important differences between species were also found regarding oxidative stress balance. In S. brachiata, the amount of flavonoids and other phenolic compounds increased in presence of NaCl, whereas these metabolites were down regulated in S. portulacastrum, who accumulated carotenoids. Furthermore, distinct impairment of membrane lipids, hormones, alkaloids and terpenes was observed in our species under salinity. Finally, several other nitrogen containing compounds were involved in response to salinity, including amino acids, serotonin and polyamine conjugates. In conclusion, metabolomics highlighted that the specific mechanism each species adopted to achieve acclimation to salinity differed in the three halophytes considered, although response osmotic stress and oxidative imbalance have been confirmed as the key processes underlying NaCl tolerance.

Keywords: Osmolytes; Oxidative stress; Plant abiotic stress; Salicornia; Sesuvium; Suaeda.