Carbon electrodes have gained wide popularity in biosensing applications in recent years. In this paper, we discuss carbon nanospikes fabricated using plasma enhanced chemical vapor deposition on a silicon wafer. Carbon nanospikes are preferred over other carbon nanostructures due to their batch reproducibility. Scanning electron microscope and Raman spectroscopy demonstrate spike-like and defectrich structure of the electrodes. Hydrogen peroxide has been chosen as the sensing analyte since it plays a vital role in various neurological disease states and is a byproduct of various electrochemical reactions.