Cell Attachment on Inside-Outside Surface and Cell Encapsulation in Wall of Microscopic Tubular Scaffolds for Vascular Tissue-Like Formation

Annu Int Conf IEEE Eng Med Biol Soc. 2018 Jul:2018:4198-4201. doi: 10.1109/EMBC.2018.8513248.

Abstract

By using the microfluidic spinning technology we generated tiny hydrogel tubular scaffolds. Fibroblast (NIH/3T3) cell cultures were performed for seventeen days to demonstrate the potential of cell attachment on surfaces and encapsulation in the wall of he microscopic scaffolds for blood vessel-like structure forming. Over theculture period, the NIH/3T3 confluence reached around 80\%, and 100\% on the inside and outside scaffolds' surface respectively while cells proliferated and coalesced in cell group in the hydrogel wall. These results could further be applied to endothelial co-culturing for forming engineered blood vessel.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Coculture Techniques
  • Humans
  • Male
  • Mice
  • Tissue Engineering*
  • Tissue Scaffolds*