Tetrodotoxin and its analogues profile in nemertean species from the sea of Japan

A E Vlasenko; P V Velansky; A V Chernyshev; V G Kuznetsov; T Yu Magarlamov

doi:10.1016/j.toxicon.2018.11.006

Tetrodotoxin and its analogues profile in nemertean species from the sea of Japan

Toxicon. 2018 Dec 15:156:48-51. doi: 10.1016/j.toxicon.2018.11.006. Epub 2018 Nov 12.

Authors

A E Vlasenko¹, P V Velansky¹, A V Chernyshev², V G Kuznetsov³, T Yu Magarlamov⁴

Affiliations

¹ National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia.
² National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia; School of Natural Sciences, Far Eastern Federal University, Vladivostok, 690092, Russia.
³ School of Biomedicine, Far Eastern Federal University, Vladivostok, 690092, Russia.
⁴ National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia. Electronic address: biotimur@yandex.ru.

PMID: 30439440
DOI: 10.1016/j.toxicon.2018.11.006

Abstract

For the first time search for tetrodotoxin (TTX) and its analogues in the extracts of nemerteans using HPLC-MS/MS was performed. TTX analogues were detected in two nemertean species in addition to TTX: 7 analogues were detected in the extract of Cephalothrix simula, 3 analogues - in the extract 11-norTTX of Kulikovia manchenkoi. Presence of 5-deoxyTTX, 11-deoxyTTX, 5,6,11-trideoxyTTX and -6(R)-ol in nemerteans was shown for the first time.

Keywords: HPLC-MS/MS; Nemerteans; TTX; Tetrodotoxin; Tetrodotoxin analogues.

MeSH terms

Animals
Aquatic Organisms / chemistry*
Aquatic Organisms / metabolism
Chromatography, High Pressure Liquid / methods
Invertebrates / chemistry
Invertebrates / metabolism
Japan
Pacific Ocean
Tandem Mass Spectrometry / methods
Tetrodotoxin / analogs & derivatives*
Tetrodotoxin / analysis*
Tetrodotoxin / chemistry
Tetrodotoxin / metabolism

Substances

Tetrodotoxin