Kreutzer, A, Martinez, CA, Kreutzer, M, Stone, JD, Mitchell, JB, and Oliver, JM. Effect of ACTN3 polymorphism on self-reported running times. J Strength Cond Res 33(1): 80-88, 2019-This investigation examined the effect of ACTN3 genotype on self-reported distance running personal records (PRs). Of 94 (n = 94) recreationally active men and women, 82 (f = 42, m = 40; age: 22.6 ± 4.5 years; body mass index [BMI]: 23.5 ± 3.4 kg·m) reported 1-mile running PRs, whereas 57 (f = 33, m = 24; age: 23.4 ± 5.3 years; BMI: 22.9 ± 9.3 kg·m) reported 5K running PRs. Subjects were grouped by the presence (ACTN3) or absence (ACTN3) of α-actinin-3, as well as by individual genotype (RR, RX, and XX). Among female participants, ACTN3 reported 64.5 seconds faster (p = 0.048) 1-mile PRs compared with their ACTN3 counterparts. No differences were observed when comparing 5K PRs between genotypes. Two one-sided test equivalence testing revealed that none of the effects observed when comparing ACTN3 and ACTN3 were equivalent to zero. Our study confirms a reportedly greater prevalence of XX benefits for endurance performance in females when compared with males but fails to strongly link ACTN3 genotype to endurance performance. Practitioners should continue to be cautious when using genetic information for talent identification and sport selection.