Colorimetric detection of gallic acid based on the enhanced oxidase-like activity of floral-like magnetic Fe3 O4 @MnO2

Luminescence. 2019 Feb;34(1):55-63. doi: 10.1002/bio.3576. Epub 2018 Nov 14.

Abstract

In this study, a colorimetric method was developed for rapid and sensitive determination of gallic acid (GA) by using floral-like magnetic Fe3 O4 @MnO2 composite material with enhanced oxidase-like activity. Fe3 O4 @MnO2 composite material is able to oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to a blue product (oxTMB) with apparent color change and absorbance at 652 nm. GA can reduce the oxTMB yielding a fading blue color. Based on these results, a technique is proposed to detect GA quantitatively and qualitatively with UV-vis spectroscopy and bare eyes. A low detection limit of 0.105 μM and a detection range of 0.01 to 15 μM were obtained with UV-vis spectroscopy. This methodology possesses high potential for application in determination of GA.

Keywords: colorimetric detection; floral-like magnetic Fe3O4@MnO2; gallic acid; nanozymes; oxidase.

MeSH terms

  • Benzidines / chemistry
  • Calibration
  • Colorimetry / methods*
  • Ferrous Compounds / chemistry*
  • Gallic Acid / analysis*
  • Gallic Acid / chemistry
  • Kinetics
  • Limit of Detection
  • Magnetics
  • Manganese Compounds / chemistry*
  • Microscopy, Electron, Transmission
  • Oxidation-Reduction
  • Oxides / chemistry*
  • Oxidoreductases / chemistry
  • Spectrophotometry, Ultraviolet
  • Water / analysis
  • X-Ray Diffraction

Substances

  • Benzidines
  • Ferrous Compounds
  • Manganese Compounds
  • Oxides
  • Water
  • 3,3',5,5'-tetramethylbenzidine
  • Gallic Acid
  • Oxidoreductases
  • ferrous oxide
  • manganese dioxide