A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols

Methods Mol Biol. 2019:1855:303-313. doi: 10.1007/978-1-4939-8793-1_26.

Abstract

The potential of capillary electrophoresis-mass spectrometry (CE-MS) for metabolomics is demonstrated through the analysis of metabolites from human HT29 colon cancer cells treated and non-treated with dietary polyphenols. Prior to CE-MS analysis, four different metabolite purification strategies are investigated. Namely, the results obtained after methanol deproteinization, ultrafiltration, and two solid-phase extraction methods using C18 and polymer-based cartridges are described. These generic methods can have broad applications to analyze metabolites in a large variety of matrices and fields, including the new Foodomics area.

Keywords: CE; CE-MS; Colon cancer; Dietary polyphenols; Foodomics; Metabolomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Colonic Neoplasms / metabolism*
  • Colonic Neoplasms / therapy
  • Electrophoresis, Capillary / methods*
  • Functional Food / analysis
  • HT29 Cells
  • Humans
  • Mass Spectrometry / methods*
  • Metabolome / drug effects*
  • Metabolomics / methods*
  • Polyphenols / pharmacology*
  • Solid Phase Extraction / methods

Substances

  • Polyphenols