Data on the effect of Angiotensin II and 6-hydroxydopamine on reactive oxygen species production, antioxidant gene expression and viability of different neuronal cell lines

Juan A Parga; Ana I Rodriguez-Perez; Maria Garcia-Garrote; Jannette Rodriguez-Pallares; Jose L Labandeira-Garcia

doi:10.1016/j.dib.2018.10.069

Data on the effect of Angiotensin II and 6-hydroxydopamine on reactive oxygen species production, antioxidant gene expression and viability of different neuronal cell lines

Data Brief. 2018 Oct 25:21:934-942. doi: 10.1016/j.dib.2018.10.069. eCollection 2018 Dec.

Authors

Juan A Parga^{1

2}, Ana I Rodriguez-Perez^{1

2}, Maria Garcia-Garrote^{1

2}, Jannette Rodriguez-Pallares^{1

2}, Jose L Labandeira-Garcia^{1

2}

Affiliations

¹ Research Center for Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
² Networking Research Center on Neurodegenerative Diseases (CIBERNED), Madrid, Spain.

Abstract

This article describes the effect of the oxidative stress inducers Angiotensin II and 6-hydroxydopamine (6-OHDA) on different cell lines. The levels of expression Angiotensin type 1 and type 2 receptors in different dopaminergic cell lines are shown. The data indicate that treatment with Angiotensin II and 6-OHDA increases the production of reactive oxygen species (ROS) and decreases cell viability. NRF2 is a transcription factor induced by ROS. We provide data that NRF2 overexpression increases cell viability in response to oxidative stress inducers compared to control cells, and that these inducers can, both separately and in combination, enhance the expression of NRF2-regulated genes heme oxygenase 1 (Hmox1), NAD(P)H quinone dehydrogenase 1 (Nqo1) and Kruppel like factor 9 (Klf9). Interpretation of these data and additional information is presented in the research article "Angiotensin II induces oxidative stress and upregulates neuroprotective signaling from the NRF2 and KLF9 pathway in dopaminergic cells" (Parga et al., 2018) [1].

Keywords: 6-OHDA, 6-hydroxydopamine; AT1, angiotensin type 1 receptor; AT2, angiotensin type 2 receptor; AngII, angiotensin II; DCFDA, 2′,7′-dichlorofluorescin-diacetate; Dopaminergic; H2O2, hydrogen peroxide; Hmox1, heme oxygenase 1; KLF9; KLF9, Kruppel like factor 9; MTT, methylthiazolyldiphenyl-tetrazolium; NAC, N-Acetyl-L-Cysteine; NRF2; NRF2, nuclear factor-E2-related factor 2; Nqo1, NAD(P)H quinone dehydrogenase 1; Oxidative stress; ROS, reactive oxygen species; Redox signaling; Renin-angiotensin system; pLV-KLF9, pLV-SV40-KLF9-puro; pLV-empty, pLV-SV40-puro.