A Simple Imaging Device for Fluorescence-Relevant Applications

Shih-Jie Lo; Chen-Meng Kuan; Min-Wei Hung; Yun Fu; J Andrew Yeh; Da-Jeng Yao; Chao-Min Cheng

doi:10.3390/mi9080418

A Simple Imaging Device for Fluorescence-Relevant Applications

Micromachines (Basel). 2018 Aug 20;9(8):418. doi: 10.3390/mi9080418.

Authors

Shih-Jie Lo¹, Chen-Meng Kuan², Min-Wei Hung³, Yun Fu⁴, J Andrew Yeh⁵, Da-Jeng Yao⁶, Chao-Min Cheng⁷

Affiliations

¹ Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan. roxyjay0406@gmail.com.
² Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan. kcmeng30@gmail.com.
³ Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 30013, Taiwan. niiat690310@gmail.com.
⁴ Department of Dermatology, Chang Gung Memorial Hospital Linkou Medical Center, Taoyuan 33305, Taiwan. amyjoy791126@hotmail.com.
⁵ Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan. jayeh@mx.nthu.edu.tw.
⁶ Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan. djyao@mx.nthu.edu.tw.
⁷ Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan. chaomin@mx.nthu.edu.tw.

Abstract

This article unveiled the development of an inexpensive, lightweight, easy-to-use, and portable fluorescence imaging device for paper-based analytical applications. We used commercial fluorescent dyes, as proof of concept, to verify the feasibility of our fluorescence imaging device for bioanalysis. This approach may provide an alternative method for nucleotide detection and semen analysis, using a miniaturized fluorescence reader that is more compact and portable than conventional analytical equipment.

Keywords: cellphone; fluorescence imaging device; nucleotide analysis; sperm analysis.