Strontium functionalized scaffold for bone tissue engineering

Mater Sci Eng C Mater Biol Appl. 2019 Jan 1:94:509-515. doi: 10.1016/j.msec.2018.09.054. Epub 2018 Sep 25.

Abstract

Drug functionalized scaffolds are currently being employed to improve local delivery of osteoprotective drugs with the aim of reducing their loading dose as well as unwanted systemic complications. In this study we tested a poly-(ε) caprolactone (PCL)-laponite-strontium ranelate (SRA) composite scaffold (PLS3) for its abilities to support growth and osteogenic differentiation of human marrow derived stromal stem cells (hMSC). The in vitro experiments showed the PLS3 scaffold supported cell growth and osteogenic differentiation. The in vivo implantation of hMSC seeded PLS3 scaffold in immunocompromised mice revealed vascularized ectopic bone formation. PLS3 scaffolds can be useful in bone regenerative applications in the fields of orthopaedics and dentistry.

Keywords: Bone; Drug delivery; Laponite; Stem cells; Strontium.

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Bone and Bones / drug effects
  • Bone and Bones / physiology*
  • Calcification, Physiologic / drug effects
  • Cell Adhesion / drug effects
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Gene Expression Regulation / drug effects
  • Humans
  • Implants, Experimental
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / enzymology
  • Mesenchymal Stem Cells / ultrastructure
  • Mice, Inbred NOD
  • Osteoblasts / cytology
  • Osteoblasts / drug effects
  • Osteogenesis / drug effects
  • Osteogenesis / genetics
  • Strontium / pharmacology*
  • Tissue Engineering
  • Tissue Scaffolds / chemistry*

Substances

  • Alkaline Phosphatase
  • Strontium