Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry

David Rojo; Coral Barbas; Ángeles López-Gonzálvez

doi:10.1007/978-1-4939-8757-3_14

Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry

Methods Mol Biol. 2019:1859:253-260. doi: 10.1007/978-1-4939-8757-3_14.

Authors

David Rojo¹, Coral Barbas¹, Ángeles López-Gonzálvez²

Affiliations

¹ Centro de Metabolómica y Bioanállisis (CEMBIO), Facultad de Farmacia, Universidad CEU San Pablo, Campus Montepríncipe, Madrid, Spain.
² Centro de Metabolómica y Bioanállisis (CEMBIO), Facultad de Farmacia, Universidad CEU San Pablo, Campus Montepríncipe, Madrid, Spain. alopgon@ceu.es.

PMID: 30421234
DOI: 10.1007/978-1-4939-8757-3_14

Abstract

Capillary electrophoresis coupled to mass spectrometry is an analytical platform ideal for the analysis of ionic or polar metabolites. It constitutes a perfect complement to reversed-phase liquid chromatography, offering a good alternative to polar stationary phases where reproducibility is not guaranteed. Herein, we describe a robust standardized methodology for the fingerprinting analysis of Leishmania, a taxonomic genus which comprises more than 20 protozoa species.

Keywords: Fingerprinting; Parasite phenotyping; Polar metabolites; Untargeted metabolomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid / instrumentation
Chromatography, High Pressure Liquid / methods
Chromatography, Reverse-Phase / instrumentation
Chromatography, Reverse-Phase / methods
Electrophoresis, Capillary / instrumentation
Electrophoresis, Capillary / methods
Leishmania / metabolism*
Metabolomics / instrumentation
Metabolomics / methods*
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization / instrumentation
Spectrometry, Mass, Electrospray Ionization / methods*