Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5

Ao Xuan Zhen; Mei Jing Piao; Yu Jae Hyun; Kyoung Ah Kang; Yea Seong Ryu; Suk Ju Cho; Hee Kyoung Kang; Young Sang Koh; Mee Jung Ahn; Tae Hoon Kim; Jin Won Hyun

doi:10.4062/biomolther.2018.151

Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5

Biomol Ther (Seoul). 2019 Jul 1;27(4):395-403. doi: 10.4062/biomolther.2018.151.

Authors

Affiliations

¹ Jeju National University School of Medicine and Jeju Research Center for Natural Medicine, Jeju 63243, Republic of Korea.
² Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju 63243, Republic of Korea.
³ Department of Food Science and Biotechnology, Daegu University, Gyeongsan 38453, Republic of Korea.

Abstract

Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 (PM_2.5) was shown to trigger excessive production of reactive oxygen species. In this study, we observed that UVB radiation and PM_2.5 severely damaged human HaCaT keratinocytes, disrupting cellular DNA, lipids, and proteins and causing mitochondrial depolarization. Purpurogallin protected HaCaT cells from apoptosis induced by UVB radiation and/or PM_2.5. Furthermore, purpurogallin effectively modulates the pro-apoptotic and anti-apoptotic proteins under UVB irradiation via caspase signaling pathways. Additionally, purpurogallin reduced apoptosis via MAPK signaling pathways, as demonstrated using MAPK-p38, ERK, and JNK inhibitors. These results indicate that purpurogallin possesses antioxidant effects and protects cells from damage and apoptosis induced by UVB radiation and PM_2.5.

Keywords: Human HaCaT keratinocytes; Oxidative stress; Particulate matter 2.5; Purpurogallin; Ultraviolet B radiation.