Evaluation of inhibitory effect and feasible utilization of dilute acid-pretreated rice straws on succinate production by metabolically engineered Escherichia coli AS1600a

Surawee Jampatesh; Apichai Sawisit; Nonthaporn Wong; Sirima Suvarnakuta Jantama; Kaemwich Jantama

doi:10.1016/j.biortech.2018.11.002

Evaluation of inhibitory effect and feasible utilization of dilute acid-pretreated rice straws on succinate production by metabolically engineered Escherichia coli AS1600a

Bioresour Technol. 2019 Feb:273:93-102. doi: 10.1016/j.biortech.2018.11.002. Epub 2018 Nov 2.

Authors

Surawee Jampatesh¹, Apichai Sawisit¹, Nonthaporn Wong¹, Sirima Suvarnakuta Jantama², Kaemwich Jantama³

Affiliations

¹ Metabolic Engineering Research Unit, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
² Division of Biopharmacy, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.
³ Metabolic Engineering Research Unit, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. Electronic address: kaemwich@sut.ac.th.

PMID: 30419446
DOI: 10.1016/j.biortech.2018.11.002

Abstract

This work demonstrated a pioneer work in the pre-treatment of rice straw by phosphoric acid (H₃PO₄) for succinate production. The optimized pre-treatment condition of rice straw was at 121 °C for 30 min with 2 N H₃PO₄. With this condition, total sugar concentration of 31.2 g/L with the highest hemicellulose saccharification yield of 94% was obtained. The physicochemical analysis of the pre-treated rice straw showed significant changes in its structure thus enhancing enzymatic saccharification. Succinate concentrations of 78.5 and 63.8 g/L were produced from hydrolysate liquor (L) and solid fraction (S) of the pre-treated rice straw respectively, with a comparable yield of 86% by E. coli AS1600a. Use of a combined L + S fraction in simultaneous saccharification and fermentation (LS + SSF) further improved succinate production at a concentration and yield of 85.6 g/L and 90% respectively. The results suggested that H₃PO₄ pre-treated rice straw may be utilized for economical succinate production by E. coli AS1600a.

Keywords: Dilute acid-pretreatment; Escherichia coli; Rice straw; Simultaneous saccharification and fermentation; Succinic acid.

MeSH terms

Acids
Escherichia coli / metabolism*
Fermentation
Hydrolysis
Indicator Dilution Techniques
Oryza / metabolism*
Succinic Acid / metabolism*

Substances

Acids
Succinic Acid