Common Cell Lines Used to Study Bone Morphogenetic Proteins (BMPs)

Jessica Ann Cottrell; Donya Burgess; Sarah Michaels; Melissa B Rogers

doi:10.1007/978-1-4939-8904-1_1

Common Cell Lines Used to Study Bone Morphogenetic Proteins (BMPs)

Methods Mol Biol. 2019:1891:1-8. doi: 10.1007/978-1-4939-8904-1_1.

Authors

Jessica Ann Cottrell¹, Donya Burgess², Sarah Michaels², Melissa B Rogers³

Affiliations

¹ Biological Sciences, Seton Hall University, South Orange, NJ, USA. Jessica.cottrell@shu.edu.
² Biological Sciences, Seton Hall University, South Orange, NJ, USA.
³ Microbiology, Biochemistry, and Molecular Genetics, Rutgers New Jersey Medical School, Newark, NJ, USA.

PMID: 30414121
DOI: 10.1007/978-1-4939-8904-1_1

Abstract

Many research methods exist to elucidate the functions of BMPs during osteogenesis. This chapter briefly reviews common immortalized mesenchymal cell types used to measure the efficacy of osteogenic factors like BMP-2. Detailed information regarding media and culture conditions are provided. Parameters relevant to experimental reproducibility and cell line authentication are discussed.

Keywords: BMP-2; Mycoplasma.

MeSH terms

Animals
Bone Morphogenetic Protein 2 / genetics
Bone Morphogenetic Protein 2 / metabolism
Bone Morphogenetic Proteins / genetics*
Bone Morphogenetic Proteins / metabolism
Cell Differentiation / genetics
Cell Line
Cells, Cultured
Humans
Osteoblasts / cytology
Osteoblasts / metabolism
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Transforming Growth Factor beta / genetics
Transforming Growth Factor beta / metabolism

Substances

Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins
Recombinant Proteins
Transforming Growth Factor beta
recombinant human bone morphogenetic protein-2