A comparative analysis of the metaphase karyotypes of Aedes excrucians, Ae. behningi, and Ae. euedes (Diptera: Culicidae) imaginal disсs

J Vector Ecol. 2018 Dec;43(2):245-251. doi: 10.1111/jvec.12308.

Abstract

Karyotypes of Aedes (Culicidae) mosquitoes (Ae. excrucians, Ae. behningi, and Ae. euedes) have been analyzed using the metaphase chromosomes of imaginal discs. Lacto-aceto-orcein, C-banding, and DAPI staining have detected species-specific features in the morphology and lengths of these chromosomes in the examined species. Species-specific features of chromosome 1 in the location of heterochromatin blocks have been shown. Thus, the metaphase chromosomes in the imaginal discs of Ae. excrucians, Ae. behningi, and Ae. euedes are a characteristic for species identification of mosquito species.

Keywords: Aedes; Blood-sucking mosquitoes; C-banding; Culicidae; DAPI; heterochromatin; imaginal discs.; lacto-aceto-orcein; mitotic chromosomes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes / genetics*
  • Aedes / parasitology
  • Aedes / physiology
  • Animals
  • Chromosomes, Insect / genetics*
  • Karyotype
  • Karyotyping
  • Malaria / transmission*
  • Metaphase
  • Mosquito Vectors / genetics*
  • Mosquito Vectors / parasitology
  • Mosquito Vectors / physiology
  • Plasmodium / physiology*
  • Species Specificity