Simple exponential decaying functions are commonly used for fitting the kinetics of starch digested by amylolytic enzymes. A common assumption is that a sole exponential function can account for the kinetics of the whole digestible starch. Recent studies using logarithm-of-slope (LOS) plots showed that digestion kinetics can exhibit multi-scale behavior, an effect reflecting starch fractions with different digestion characteristics. This work proposed an extension of the widely used Goñi et al.'s model to account for two starch fractions; one fraction linked with fast digestion rate and other with slow digestion rates. The fitting of experimental data was carried out by solving numerically a nonlinear least-squares problem. The estimated parameters have a straightforward interpretation in terms of reaction rates and digestible/resistant starch fractions. Two experimental examples were used for illustrating the performance of the multi-exponential function.
Keywords: Amylases; Digestion; Kinetics; Model; Starch.
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