Cloning, expression prolife, and immune characterization of a novel stat family member (stat5bl) in Chinese tongue sole (Cynoglossus semilaevis)

Min Wei; Wen-Teng Xu; Tian Gan; Lei Wang; Hong-Xiang Zhang; Fa-Zhen Zhao; Song-Lin Chen

doi:10.1016/j.fsi.2018.10.030

Cloning, expression prolife, and immune characterization of a novel stat family member (stat5bl) in Chinese tongue sole (Cynoglossus semilaevis)

Fish Shellfish Immunol. 2019 Jan:84:962-969. doi: 10.1016/j.fsi.2018.10.030. Epub 2018 Nov 3.

Authors

Min Wei¹, Wen-Teng Xu², Tian Gan², Lei Wang², Hong-Xiang Zhang², Fa-Zhen Zhao², Song-Lin Chen³

Affiliations

¹ Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China; Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Science and Fisheries, Huaihai Institute of Technology, Lianyungang, 222005, China.
² Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
³ Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao, 266071, China. Electronic address: chensl@ysfri.ac.cn.

PMID: 30399402
DOI: 10.1016/j.fsi.2018.10.030

Abstract

STAT plays important roles in innate immunity during JAK/STAT signaling pathway, and STAT5 is particularly focused due to the existence of duplicated forms in fish and mammal. In Chinese tongue sole, stat5bl was suggested to be a candidate related to Vibrio harveyi resistance based on previous QTL screening. In this study, the full length of stat5bl cDNA was cloned and its expression patterns were analyzed. stat5bl was predominantly expressed in immune tissues, where the highest level was observed in liver, followed by skin and gill. Time course expression patterns were examined in six tissues (liver, skin, gill, kidney, intestine, spleen) after V. harveyi infection. stat5bl could be up-regulated by V. harveyi infection in all tissues except liver, despite the timepoints of peak were different. In contrast, stat5bl was significantly downregulated in liver. To elucidate the role of stat5bl in liver, in vitro RNAi were performed using primary liver cell culture. Knockdown of stat5bl could regulate the expression of genes closely related to JAK/STAT pathway. This study would enlarge our understanding of stat5bl in fish immunity.

Keywords: Chinese tongue sole; Expression pattern; Immunity; Vibrio harveyi; stat5b-like.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Fish Diseases / immunology*
Fish Proteins / chemistry
Fish Proteins / genetics
Fish Proteins / immunology
Flatfishes / genetics*
Flatfishes / immunology*
Gene Expression Profiling / veterinary
Gene Expression Regulation / immunology*
Immunity, Innate / genetics*
Phylogeny
STAT5 Transcription Factor / chemistry
STAT5 Transcription Factor / genetics*
STAT5 Transcription Factor / immunology*
Sequence Alignment / veterinary
Vibrio
Vibrio Infections

Substances

Fish Proteins
STAT5 Transcription Factor