Microsatellite Typing and Resistance Mechanism Analysis of Voriconazole-Resistant Aspergillus flavus Isolates in South Korean Hospitals

Antimicrob Agents Chemother. 2019 Jan 29;63(2):e01610-18. doi: 10.1128/AAC.01610-18. Print 2019 Feb.

Abstract

A recent surveillance study in South Korea revealed that 14% (7/50) of Aspergillus flavus clinical isolates had a voriconazole minimum inhibitory concentration of ≥4 μg/ml. Of seven non-wild-type (non-WT) isolates, six ear isolates from four hospitals shared the same microsatellite genotype. None of the non-WT isolates showed cyp51 mutations associated with azole resistance. However, the mean expression levels of efflux pump (MDR2, atrF, and mfs1) and target (cyp51A) genes exhibited significant differences between non-WT and other isolates.

Keywords: Aspergillus flavus; azole resistance mechanism; gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B / genetics
  • ATP-Binding Cassette Sub-Family B Member 4
  • Antifungal Agents / pharmacology*
  • Aspergillus flavus / drug effects*
  • Aspergillus flavus / genetics*
  • Drug Resistance, Fungal / genetics
  • Genotype
  • Hospitals
  • Humans
  • Microbial Sensitivity Tests
  • Microsatellite Repeats / genetics*
  • Mutation / genetics
  • Republic of Korea
  • Voriconazole / pharmacology*

Substances

  • ATP Binding Cassette Transporter, Subfamily B
  • Antifungal Agents
  • Voriconazole