Enhancement of Monascus pigment productivity via a simultaneous fermentation process and separation system using immobilized-cell fermentation

Jun Liu; Ting Guo; Yunchuan Luo; Xueying Chai; Jingyan Wu; Wen Zhao; Pengfei Jiao; Feijun Luo; Qinlu Lin

doi:10.1016/j.biortech.2018.10.072

Enhancement of Monascus pigment productivity via a simultaneous fermentation process and separation system using immobilized-cell fermentation

Bioresour Technol. 2019 Jan:272:552-560. doi: 10.1016/j.biortech.2018.10.072. Epub 2018 Oct 29.

Authors

Jun Liu¹, Ting Guo¹, Yunchuan Luo¹, Xueying Chai¹, Jingyan Wu¹, Wen Zhao¹, Pengfei Jiao², Feijun Luo¹, Qinlu Lin³

Affiliations

¹ Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, Hunan Key Laboratory of Processed Food for Special Medical Purpose, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha, Hunan 410004, China.
² School of Life Science and Technology, Nanyang Normal University, No. 1638 Wolong Road, Nanyang, Henan 473061, China.
³ Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, Hunan Key Laboratory of Processed Food for Special Medical Purpose, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha, Hunan 410004, China. Electronic address: linqinlu@hotmail.com.

PMID: 30396112
DOI: 10.1016/j.biortech.2018.10.072

Abstract

A mutant Monascus purpureus strain, M183, which produced monascus pigments (MPs) at 8460 U/g via solid-state batch-fermentation, was generated using the atmospheric and room temperature plasma (ARTP) mutation system. The optimal glucose concentration (80 g/L) in traditional fermentation media that yielded the highest MPs productivity was determined. Response surface methodology (RSM) was applied to maximize MPs production using liquid-state batch-fermentation. Under optimal conditions (0.58 g/L MgSO₄·7H₂O, 0.02 g/L ZnSO₄·7H₂O, 0.02 g/L FeSO₄·7H₂O and 4.85 g/L NH₄NO₃), 207.67 U/mL of MPs were produced with 98.12% validity based on the predicted value. Extracellular MPs production increased significantly to 35.52 U/mL, compared to 14.19 U/mL of the original strain, M. purpureus LQ-6. M. purpureus spores immobilized in sodium alginate were studied. A simultaneous fermentation and separation system was established for MPs using the novel absorption resin LX300C to enhance production efficiency of extracellular MPs.

Keywords: Immobilized-cells; Monascus pigments; Response surface methodology; Simultaneous fermentation process and separation.

MeSH terms

Fermentation*
Monascus / metabolism*
Pigments, Biological / metabolism*

Substances

Pigments, Biological