The detection of canine circovirus in Guangxi, China

Wenchao Sun; Hongyun Zhang; Min Zheng; Huihui Cao; Huijun Lu; Guanyu Zhao; Changzhan Xie; Liang Cao; Xiankai Wei; Jingshan Bi; Chizhe Yi; Gefen Yin; Ningyi Jin

doi:10.1016/j.virusres.2018.10.021

The detection of canine circovirus in Guangxi, China

Virus Res. 2019 Jan 2:259:85-89. doi: 10.1016/j.virusres.2018.10.021. Epub 2018 Nov 2.

Authors

Wenchao Sun¹, Hongyun Zhang², Min Zheng³, Huihui Cao⁴, Huijun Lu⁵, Guanyu Zhao⁵, Changzhan Xie⁵, Liang Cao⁵, Xiankai Wei⁴, Jingshan Bi⁴, Chizhe Yi⁶, Gefen Yin⁷, Ningyi Jin⁸

Affiliations

¹ Institute of Virology, Wenzhou University, Wenzhou 325035, China; Guangxi Center for Animal Disease Control and Prevention, Nanning, 530001, China; Institute of Military Veterinary, The Academy of Military Medical Sciences, Changchun 130122, China.
² Yulin Center for Animal Disease Control and Prevention, Yulin 537000, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
³ Guangxi Center for Animal Disease Control and Prevention, Nanning, 530001, China. Electronic address: zhgmn26@163.com.
⁴ Guangxi Center for Animal Disease Control and Prevention, Nanning, 530001, China.
⁵ Institute of Military Veterinary, The Academy of Military Medical Sciences, Changchun 130122, China.
⁶ College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
⁷ College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
⁸ Institute of Military Veterinary, The Academy of Military Medical Sciences, Changchun 130122, China. Electronic address: ningyik@126.com.

PMID: 30395895
DOI: 10.1016/j.virusres.2018.10.021

Abstract

Since the first description of canine circovirus (CanineCV)-associated infection, there have been several reports on the distribution of the disease in worldwide. To investigate the prevalence and genetic diversity of CanineCV in China, we conducted PCR screening of 1226 dog serum samples collected from different regions in mainland China between 2014 and 2016. CanineCV DNA was found in 81/926 serum samples from Guangxi Province. Furthermore, 25 full-length genomes of CanineCV from positive samples were sequenced and compared with CanineCV sequences in the GenBank database. Pairwise analysis showed that the determined genome sequences shared 84.9%-100% identity among themselves and 81.4%-90.5% with the other 28 sequences. Phylogenetic analysis revealed that the 52 viral genome sequences could be divided into two genotypes (CanineCV-1 and CanineCV-2). Analysis of the amino acid sequences of the capsid protein revealed the existence of 9 major regions of variation. The present work contributes to the understanding of CanineCV molecular epidemiology.

Keywords: Canine circovirus; Epidemiology; Phylogenetic analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
China / epidemiology
Circoviridae Infections / veterinary*
Circovirus / classification*
Circovirus / genetics*
Dog Diseases / epidemiology*
Dog Diseases / virology*
Dogs
Genome, Viral
Genomics / methods
Open Reading Frames
Phylogeny
Recombination, Genetic