Introducing nano-scale quantitative polymerase chain reaction

Sissel E Dyrstad; Deusdedit Tusubira; Stian Knappskog; Karl J Tronstad; Gro V Røsland

doi:10.1016/j.bbrc.2018.10.111

Introducing nano-scale quantitative polymerase chain reaction

Biochem Biophys Res Commun. 2018 Dec 2;506(4):923-926. doi: 10.1016/j.bbrc.2018.10.111. Epub 2018 Nov 2.

Authors

Sissel E Dyrstad¹, Deusdedit Tusubira¹, Stian Knappskog², Karl J Tronstad¹, Gro V Røsland³

Affiliations

¹ Department of Biomedicine, University of Bergen, Norway.
² Department of Clinical Science, University of Bergen, Bergen, Norway; Department of Oncology, Haukeland University Hospital, Bergen, Norway.
³ Department of Biomedicine, University of Bergen, Norway; Department of Oncology, Haukeland University Hospital, Bergen, Norway. Electronic address: gro.rosland@uib.no.

PMID: 30392909
DOI: 10.1016/j.bbrc.2018.10.111

Abstract

We show that the quantitative PCR reaction volume can be reduced significantly compared to the standard procedures, without compromising data quality. By analyzing dilution series (10⁰-10^-7) we found that measurement in 1 μl reaction volume indeed gave valid results comparable to 10 μL, when using a routine pipetting robot. This may enable a significant cost reduction through cutback of both biological material as well as chemical reagents.

Keywords: Nano-scale qPCR; TaqMan technology; qPCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Humans
Nanotechnology / methods*
Real-Time Polymerase Chain Reaction / methods*
Reference Standards