Hemin-incorporated nanoflowers as enzyme mimics for colorimetric detection of foodborne pathogenic bacteria

Bioorg Med Chem Lett. 2018 Dec 15;28(23-24):3802-3807. doi: 10.1016/j.bmcl.2018.07.017. Epub 2018 Jul 10.

Abstract

Rapid, sensitive and point-of-care detection of foodborne pathogenic bacteria is essential for food safety. In this study, we found that hemin-concanavalin A hybrid nanoflowers (HCH nanoflowers), as solid mimic peroxidase, could catalyze oxidation of 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) in the presence of H2O2 to a green-colored product. HCH nanoflowers, integrating the essential functions of both biological recognition and signal amplification, meet the requirements of signal labels for colorimetric immunoassay of bacteria. In view of the excellent peroxidase mimetic catalytic activity of HCH nanoflowers, a colorimetric biosensing platform was newly constructed and applied for sensitive detection of foodborne Escherichia coli O157:H7 (E. coli O157:H7). The corresponding detection limits was as low as 4.1 CFU/mL with wide linear ranges (101-106 CFU/mL).

Keywords: Colorimetric detection; Enzyme mimics; Hybrid nanoflowers; Pathogenic bacteria.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzothiazoles / chemistry
  • Biomimetic Materials / chemistry*
  • Biosensing Techniques / methods*
  • Colorimetry / methods*
  • Escherichia coli Infections / microbiology*
  • Escherichia coli O157 / isolation & purification*
  • Foodborne Diseases / microbiology
  • Hemin / chemistry*
  • Humans
  • Hydrogen Peroxide / chemistry
  • Immunoassay / methods
  • Limit of Detection
  • Milk / microbiology
  • Nanostructures / chemistry*
  • Peroxidase / chemistry
  • Sulfonic Acids / chemistry

Substances

  • Benzothiazoles
  • Sulfonic Acids
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • Hemin
  • Hydrogen Peroxide
  • Peroxidase