A fluorescent sensor for discrimination of HSA from BSA through selectivity evolution

Anal Chim Acta. 2018 Dec 28:1043:123-131. doi: 10.1016/j.aca.2018.09.010. Epub 2018 Sep 8.

Abstract

Pre-clinical diagnosis of many diseases required quantitative detection of Human Serum Albumin (HSA). Herein a high-selective HSA sensor RhHSA was picked through a two-round selectivity evolution from the typical "Effector-π-Trigger" style. RhHSA suggested advantages including high selective (∼6 fold for HSA:BSA = 1:10), sensitive (LOD ∼ 5 nM, over 700-fold enhancement), steady (over 24 h) and wide linear range (0-0.5 mg/mL, applicative for conventional HSA measurement). The detecting system was free from media polarity or viscosity. HSA destruction, site competition and molecular docking provided reliable evidence for the fact that RhHSA could be embedded into both ibuprofen and phenylbutazone sites of HSA. These hints also supported the discrimination of HSA from BSA. Stepwisely fluid replacement in living cells and measuring in urine system both inferred the potential of RhHSA in biological applications.

Keywords: Application; Biological imaging; Fluorescent; Human serum albumin; Selectivity evolution.

MeSH terms

  • Animals
  • Binding Sites
  • Cattle
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / metabolism
  • Humans
  • Limit of Detection
  • MCF-7 Cells
  • Microscopy, Fluorescence
  • Molecular Docking Simulation
  • Protein Binding
  • Quantum Theory
  • Rhodamines / chemistry
  • Serum Albumin, Bovine / analysis*
  • Serum Albumin, Bovine / metabolism
  • Serum Albumin, Human / analysis*
  • Serum Albumin, Human / isolation & purification
  • Serum Albumin, Human / metabolism
  • Spectrometry, Fluorescence*

Substances

  • Fluorescent Dyes
  • Rhodamines
  • Serum Albumin, Bovine
  • rhodamine B
  • Serum Albumin, Human