High-throughput screening assays have become nearly ubiquitous in the search for small compounds or peptides that can modulate biological processes for therapeutic purposes. While many assays have become quite robust, with well-established protocols, the subsequent steps of validating the hits and choosing the best ones to take forward into leads for further chemical development are less established. In this chapter, we describe a variety of approaches, including chemical assessment, the use of various computational approaches, a variety of counter-screens, and "orthogonal" biophysical assays using nuclear magnetic resonance, surface plasmon resonance, isothermal titration calorimetry or thermal shift assays as methods for validating and assessing the quality of hits.
Keywords: ADMET; Assessment; Chemical diversity; Counter-assays; Drug-likeness; Fragment molecular orbital; High-throughput screening; Hit validation; Isothermal titration calorimetry; Ligand efficiency; Molecular docking; NMR; Promiscuity; Reactivity; Rescoring; Resynthesis; Secondary assays; Surface plasmon resonance; Thermal shift assay; Toxicity; Virtual screening.
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