Evolution at the Cutting Edge: CRISPR-Mediated Directed Evolution

Timothy R Abbott; Lei S Qi

doi:10.1016/j.molcel.2018.10.027

Evolution at the Cutting Edge: CRISPR-Mediated Directed Evolution

Mol Cell. 2018 Nov 1;72(3):402-403. doi: 10.1016/j.molcel.2018.10.027.

Authors

Timothy R Abbott¹, Lei S Qi²

Affiliations

¹ Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
² Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; Stanford ChEM-H, Stanford University, Stanford, CA 94305, USA. Electronic address: stanley.qi@stanford.edu.

PMID: 30388408
DOI: 10.1016/j.molcel.2018.10.027

Abstract

In a recent issue of Nature, Halperin et al. (2018) develop a new technology to continuously diversify specific genomic loci by combining CRISPR-Cas9 with error-prone DNA polymerases.

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MeSH terms

CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats*
DNA-Directed DNA Polymerase
Genomics
Nucleotides

Substances

Nucleotides
DNA-Directed DNA Polymerase