Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins

Emerg Infect Dis. 2019 Jan;25(1):1111-1115. doi: 10.3201/eid2501.180605. Epub 2019 Jan 17.

Abstract

We developed an IgM-based ELISA that identifies the dengue virus serotype of recent infections. Dominant serotypes were detectable in 91.1% of samples from travelers and 86.5% of samples from residents of endemic regions; 97.1% corresponded to the serotype identified by PCR. This ELISA enables more accurate reporting of epidemiologic findings.

Keywords: Brazil; ELISA; Germany; IgM; Italy; Sri Lanka; Venezuela; Vietnam; cross-reactivity; dengue; dengue virus; diagnosis; diagnostics; envelope; flavivirus; fusion loop; methods; serologic test; serotypes; travelers; vector-borne infections; viruses.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral / blood*
  • Cross Reactions
  • Dengue / diagnosis
  • Dengue / epidemiology*
  • Dengue / virology
  • Dengue Virus / classification
  • Dengue Virus / genetics
  • Dengue Virus / immunology*
  • Endemic Diseases*
  • Enzyme-Linked Immunosorbent Assay
  • Germany / epidemiology
  • Humans
  • Immunoglobulin M / blood*
  • Italy / epidemiology
  • Mutant Proteins / immunology
  • Recombinant Proteins
  • Serotyping
  • Viral Envelope Proteins / immunology*

Substances

  • Antibodies, Viral
  • Immunoglobulin M
  • Mutant Proteins
  • Recombinant Proteins
  • Viral Envelope Proteins