Abstract
We developed an IgM-based ELISA that identifies the dengue virus serotype of recent infections. Dominant serotypes were detectable in 91.1% of samples from travelers and 86.5% of samples from residents of endemic regions; 97.1% corresponded to the serotype identified by PCR. This ELISA enables more accurate reporting of epidemiologic findings.
Keywords:
Brazil; ELISA; Germany; IgM; Italy; Sri Lanka; Venezuela; Vietnam; cross-reactivity; dengue; dengue virus; diagnosis; diagnostics; envelope; flavivirus; fusion loop; methods; serologic test; serotypes; travelers; vector-borne infections; viruses.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Viral / blood*
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Cross Reactions
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Dengue / diagnosis
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Dengue / epidemiology*
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Dengue / virology
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Dengue Virus / classification
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Dengue Virus / genetics
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Dengue Virus / immunology*
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Endemic Diseases*
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Enzyme-Linked Immunosorbent Assay
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Germany / epidemiology
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Humans
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Immunoglobulin M / blood*
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Italy / epidemiology
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Mutant Proteins / immunology
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Recombinant Proteins
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Serotyping
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Viral Envelope Proteins / immunology*
Substances
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Antibodies, Viral
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Immunoglobulin M
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Mutant Proteins
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Recombinant Proteins
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Viral Envelope Proteins