P38 is involved in immune response by regulating inflammatory cytokine expressions in the Pacific oyster Crassostrea gigas

Jiejie Sun; Lingling Wang; Zhaojun Wu; Shuo Han; Liyan Wang; Meijia Li; Zhaoqun Liu; Linsheng Song

doi:10.1016/j.dci.2018.10.011

P38 is involved in immune response by regulating inflammatory cytokine expressions in the Pacific oyster Crassostrea gigas

Dev Comp Immunol. 2019 Feb:91:108-114. doi: 10.1016/j.dci.2018.10.011. Epub 2018 Oct 29.

Authors

Jiejie Sun¹, Lingling Wang², Zhaojun Wu¹, Shuo Han¹, Liyan Wang¹, Meijia Li¹, Zhaoqun Liu¹, Linsheng Song³

Affiliations

¹ Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China.
² Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China.
³ Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China. Electronic address: lshsong@dlou.edu.cn.

PMID: 30385315
DOI: 10.1016/j.dci.2018.10.011

Abstract

P38 mitogen-activated protein kinases are serine/threonine protein kinases reportedly involved in the innate immunity of vertebrates and invertebrates. In the present study, a P38 homolog (CgP38) was characterized from the Pacific oyster Crassostrea gigas. The full-length cDNA of CgP38 was of 1515 bp containing a 1101 bp open reading frame. A serine/threonine protein kinase (S_TKc) domain with a conserved Thr-Gly-Tyr motif and an ATRW substrate-binding site was found in the deduced amino acid sequence of CgP38. CgP38 shared a close evolutionary relationship with ChP38 from the Hong Kong oyster Crassostrea hongkongensis. The transcript levels of CgP38 in hemocytes increased significantly from 12 h to 48 h after lipopolysaccharide (LPS) stimulation and from 12 h to 24 h after Vibrio splendidus stimulation. The phosphorylation level of CgP38 in oyster hemocytes increased significantly at 2 h after LPS stimulation. CgP38 positively regulated the expression of interleukins, such as CgIL17-1, CgIL17-2, CgIL17-3, CgIL17-4 and CgIL17-6, and tumor necrosis factor CgTNF after LPS or V. splendidus stimulation. These results suggested that CgP38 participated in oyster immune response by regulating the expressions of inflammatory cytokines.

Keywords: Interleukins; Lipopolysaccharide; Mitogen-activated protein kinase; Oysters.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Cloning, Molecular
Crassostrea / immunology*
Crassostrea / microbiology
Cytokines / metabolism
Evolution, Molecular
Hemocytes / physiology*
Immunity, Innate
Inflammation / immunology*
Lipopolysaccharides / immunology
Phosphorylation
Phylogeny
Vibrio / physiology*
Vibrio Infections / immunology*
p38 Mitogen-Activated Protein Kinases / genetics*
p38 Mitogen-Activated Protein Kinases / metabolism

Substances

Cytokines
Lipopolysaccharides
p38 Mitogen-Activated Protein Kinases