Use of Yarrowia lipolytica Lipase Immobilized in Cell Debris for the Production of Lipolyzed Milk Fat (LMF)

Int J Mol Sci. 2018 Oct 31;19(11):3413. doi: 10.3390/ijms19113413.

Abstract

Lipase immobilized on Yarrowia lipolytica cell debris after sonication of yeast cells (LipImDebri) was used in hydrolysis reaction as a novel strategy to produce lipolyzed milk fat (LMF). Extracellular (4732.1 U/L), intracellular (130.0 U/g), and cell debris (181.0 U/g) lipases were obtained in a 4 L bioreactor using residual frying oil as inducer in 24 h fermentation process. LipImDebri showed a good operational stability retaining 70% of lipolytic activity after the second cycle and 40% after the fourth. The highest degree of hydrolysis (28%) was obtained with 500 mg LipImDebri for 6 h of lipolysis of anhydrous milk fat. LMF produced with LipImDebri presented high contents of oleic (35.2%), palmitic (25.0%), and stearic (15.4%) acids and considerable amounts of odor-active short and medium chain fatty acids (C:4⁻C:10) (8.13%).

Keywords: cell debris; fatty acids; lipase; lipolyzed milk fat.

MeSH terms

  • Animals
  • Enzymes, Immobilized / chemistry*
  • Fatty Acids / chemistry
  • Fungal Proteins / chemistry*
  • Lipase / chemistry*
  • Lipolysis*
  • Milk / chemistry*
  • Yarrowia / enzymology*

Substances

  • Enzymes, Immobilized
  • Fatty Acids
  • Fungal Proteins
  • Lipase