Hydrophilic interaction liquid chromatography coupled with quadrupole-orbitrap ultra high resolution mass spectrometry to quantitate nucleobases, nucleosides, and nucleotides during white tea withering process

Feng Zhao; Xiaohong Qiu; Naixing Ye; Jiang Qian; Danghong Wang; Peng Zhou; Mingjie Chen

doi:10.1016/j.foodchem.2018.06.030

Hydrophilic interaction liquid chromatography coupled with quadrupole-orbitrap ultra high resolution mass spectrometry to quantitate nucleobases, nucleosides, and nucleotides during white tea withering process

Food Chem. 2018 Nov 15:266:343-349. doi: 10.1016/j.foodchem.2018.06.030. Epub 2018 Jun 8.

Authors

Feng Zhao¹, Xiaohong Qiu², Naixing Ye³, Jiang Qian⁴, Danghong Wang⁴, Peng Zhou⁵, Mingjie Chen⁶

Affiliations

¹ College of Horticulture and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Key Laboratory of Tea Science in Fujian Provincial University, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Horticultural Plant Biology and Metabolomics Center/FAFU-UCR Joint Center, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
² College of Horticulture and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Key Laboratory of Tea Science in Fujian Provincial University, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
³ College of Horticulture and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Key Laboratory of Tea Science in Fujian Provincial University, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: ynxtea@qq.com.
⁴ Inspection and Quarantine Technology Center of Fujian Entry-exit Inspection and Quarantine Bureau, Fuzhou, Fujian, China.
⁵ Fujian Inspection and Research Institute for Product Quality, Fuzhou, Fujian 350002, China.
⁶ College of Horticulture and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Horticultural Plant Biology and Metabolomics Center/FAFU-UCR Joint Center, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: chenmj@fafu.edu.cn.

PMID: 30381196
DOI: 10.1016/j.foodchem.2018.06.030

Abstract

Nucleotides, nucleosides, and nucleobases are important bioactive compounds. Recent studies suggested that they possess taste activity. However, it remains unknown about their presence in white tea and how they change during white tea manufacture. Here, we first established method based on hydrophilic interaction liquid chromatography coupled with quadrupole-orbitrap ultra high resolution mass spectrometry (HILIC-Quadrupole-Orbitrap-UHRMS) platform, then applied it to study the dynamic changes of nucleotides, nucleosides, and nucleobases during white tea withering process. Five compounds, including adenosine 5'-monophosphate monohydrate (AMP), guanosine 5'-monophosphate disodium salt hydrate (GMP), adenosine, cytidine, thymine and uracil, were detected from withering samples. They showed a general decline trend during white tea withering process, however, considerable amount of them was retained after withering for 48 h except adenosine which was below detection limit after withering for 21 h. This study provided a complete picture about nucleotides, nucleosides and nucleobases changes during white tea withering process.

Keywords: Adenine (PubChem CID: 190); Adenosine (PubChem CID: 60961); Adenosine 5′-monophosphate monohydrate (AMP) (PubChem CID: 6083); Cytidine (PubChem CID: 6175); Cytidine 5′-monophosphate (CMP) (PubChem CID: 6131); Cytosine (PubChem CID: 597); Guanine (PubChem CID: 764); Guanosine (PubChem CID: 6802); Guanosine 5′-monophosphate disodium salt hydrate (GMP) (PubChem CID: 21712); HILIC-Quadrupole-Orbitrap-UHRMS; Hypoxanthine (PubChem CID: 790); Inosine (PubChem CID: 6021); Inosine-5′-monophosphate disodium salt octahydrate (IMP) (PubChem CID: 8582); Nucleobases; Nucleosides; Nucleotides; Thymine (PubChem CID: 1135); Uracil (PubChem CID: 1174); Uridine (PubChem CID: 6029); Uridine 5′-monophosphate disodium salt (UMP) (PubChem CID: 6030); White tea; Withering process; Xanthine (PubChem CID: 1188).

MeSH terms

Chromatography, Liquid / methods*
Food Handling*
Hydrophobic and Hydrophilic Interactions*
Limit of Detection
Mass Spectrometry / methods*
Nucleosides / analysis*
Nucleotides / analysis*
Tea / chemistry*

Substances

Nucleosides
Nucleotides
Tea