Abstract
Polymethacrylate-copolymer (PMA) encased lipid-nanodiscs (∼10 nm) and macro-nanodiscs (>15 nm) are used to study Aβ1-40 aggregation. We demonstrate that PMA-nanodiscs form a ternary association with Aβ and regulate its aggregation kinetics by trapping intermediates. Results demonstrating the reduced neurotoxicity of nanodisc-bound Aβ oligomers are also reported.
MeSH terms
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Alzheimer Disease / metabolism
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Alzheimer Disease / pathology
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Amyloid beta-Peptides / chemistry
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Amyloid beta-Peptides / metabolism*
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Amyloid beta-Peptides / pharmacology
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Cell Line, Tumor
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Cell Survival / drug effects
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Circular Dichroism
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Dimyristoylphosphatidylcholine / chemistry
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Dynamic Light Scattering
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Humans
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Kinetics
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Microscopy, Fluorescence
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Nanostructures / chemistry*
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Peptide Fragments / chemistry
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Peptide Fragments / metabolism*
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Peptide Fragments / pharmacology
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Polymethacrylic Acids / chemistry*
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Protein Binding
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Thermodynamics
Substances
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Amyloid beta-Peptides
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Peptide Fragments
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Polymethacrylic Acids
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amyloid beta-protein (1-40)
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polymethacrylic acid
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Dimyristoylphosphatidylcholine