Aptamers (DNA or RNA) have complex three-dimensional shapes that can bind to specific targets. Relative to antibodies, aptamers benefit from their low cost of production, easy chemical modification, high chemical stability, reproducibility, and low levels of immunogenicity and toxicity. However, the true value of aptamers lies in their simplicity by which these molecules can be engineered into sensors as bio-recognition elements in diagnostics, drug discovery and therapy, environmental monitoring and food quality testing, etc. Many different types of techniques, such as optical, electrochemical, radiochemical and piezoelectronic methods, have been applied for the design of aptamer-based methods, in which chemiluminescence (CL) detection techniques have become very popular in recent years. This review focuses on the recent advances in the development of aptamer-based CL sensors for different target detection. We highlight specific examples that showcase the use of aptamers in practical applications, and provide the challenges and opportunities in this promising field.
Keywords: aptamer; aptasensor; chemiluminescence.
© 2018 John Wiley & Sons, Ltd.