Arabidopsis NITROGEN LIMITATION ADAPTATION regulates ORE1 homeostasis during senescence induced by nitrogen deficiency

Nat Plants. 2018 Nov;4(11):898-903. doi: 10.1038/s41477-018-0269-8. Epub 2018 Oct 29.

Abstract

Nitrogen is an important macronutrient in plants and its deficiency induces rapid leaf senescence. Two genes, ORE1 and NITROGEN LIMITATION ADAPTATION (NLA), have been implicated in regulating the senescence process but their relationship is unclear1,2. Here, we show that nla and pho2 (also known as ubc24) plants develop rapid leaf senescence under nitrogen-starvation condition, whereas ore1 and nla/ore1 and pho2 (ubc24)/ore1 plants stay green. These results suggest that ORE1 acts downstream of NLA and PHO2 (UBC24). NLA interacts with ORE1 in the nucleus and regulates its stability through polyubiquitination using PHO2 (UBC24) as the E2 conjugase. Our findings identified ORE1 as a downstream target of NLA/PHO2 (UBC24) and showed that post-translational regulation of ORE1 levels determines leaf senescence during nitrogen deficiency.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / metabolism
  • Aging / physiology*
  • Arabidopsis / metabolism
  • Arabidopsis / physiology*
  • Arabidopsis Proteins / metabolism*
  • Arabidopsis Proteins / physiology*
  • Gene Expression Regulation, Plant
  • Nitrogen / deficiency*
  • Plant Leaves / metabolism
  • Plant Leaves / physiology
  • Transcription Factors / metabolism*
  • Ubiquitin-Conjugating Enzymes / metabolism
  • Ubiquitin-Protein Ligases / metabolism
  • Ubiquitin-Protein Ligases / physiology*
  • Ubiquitination

Substances

  • Arabidopsis Proteins
  • ORE1 protein, Arabidopsis
  • Transcription Factors
  • UBC24 protein, Arabidopsis
  • Ubiquitin-Conjugating Enzymes
  • NLA protein, Arabidopsis
  • Ubiquitin-Protein Ligases
  • Nitrogen