Analysis of multiple pesticide residues in polyphenol-rich agricultural products by UPLC-MS/MS using a modified QuEChERS extraction and dilution method

Jiagang Guo; Mengmeng Tong; Jie Tang; Hongzheng Bian; Xiaochun Wan; Lili He; Ruyan Hou

doi:10.1016/j.foodchem.2018.08.134

Analysis of multiple pesticide residues in polyphenol-rich agricultural products by UPLC-MS/MS using a modified QuEChERS extraction and dilution method

Food Chem. 2019 Feb 15:274:452-459. doi: 10.1016/j.foodchem.2018.08.134. Epub 2018 Aug 31.

Authors

Jiagang Guo¹, Mengmeng Tong¹, Jie Tang¹, Hongzheng Bian², Xiaochun Wan¹, Lili He³, Ruyan Hou⁴

Affiliations

¹ State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects, School of Tea and Food Science & Technology, Anhui Agricultural University, Anhui Province Key Lab of Analysis and Detection for Food Safety, Hefei 230036, PR China.
² Supervision and Testing Center for Agri-products Quality and Safety, Ministry of Agriculture, Hefei 230091, PR China.
³ Department of Food Science, University of Massachusetts, Amherst, MA 01003, United States.
⁴ State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects, School of Tea and Food Science & Technology, Anhui Agricultural University, Anhui Province Key Lab of Analysis and Detection for Food Safety, Hefei 230036, PR China. Electronic address: hry@ahau.edu.cn.

PMID: 30372964
DOI: 10.1016/j.foodchem.2018.08.134

Abstract

An efficient method for the rapid extraction and clean-up of 20 pesticide residues from polyphenol-rich agricultural samples (tea, apple, broccoli, and shallot) for analysis by UPLC-MS/MS was developed. Following extraction, PVPP was used to precipitate polyphenols, supernatant was diluted to minimize matrix effects. Homogenized samples were vortexed in acetonitrile prior to cleanup with a combination of PVPP (150 mg), PSA (50 mg) and GCB (10 mg). Supernatant (1 mL) was filtered and diluted 10-fold before analysis. In 4 agricultural products that usually produce high interference, the matrix effects were overcome for all pesticides expect in green and oolong tea for acephate, omethoate, dinotefuran and nitenpyram. Mean recoveries ranged from 73% to 106%, and RSD ≦ 13%. Limits of quantification ranged from 0.01 to 0.02 mg kg^-1. PVPP as one of excellent QuEChERS material combined with dilution was verified as a promising method for multiple pesticide residues analysis in complex matrices.

Keywords: Acephate (PubChem CID: 1982); Acetamiprid (PubChem CID: 213021); Agricultural products; Buprofezin (PubChem CID: 50367); Carbendazim (PubChem CID: 25429); Chlorpyrifos (PubChem CID: 2730); Dilution; Dimethoate (PubChem CID: 3082); Imidacloprid (PubChem CID: 86418); Methamidophos (PubChem CID: 4096); Multiclass residues; PVPP; Profenofos (PubChem CID: 38779); Thiamethoxam (PubChem CID: 5821911); UPLC-MS/MS; d-SPE.

MeSH terms

Agriculture*
Chemical Fractionation / methods*
Chromatography, High Pressure Liquid
Costs and Cost Analysis*
Food Contamination / analysis
Pesticide Residues / analysis*
Pesticide Residues / isolation & purification*
Polyphenols / analysis*
Safety*
Tandem Mass Spectrometry
Time Factors

Substances

Pesticide Residues
Polyphenols