miR-513a-5p targets Bcl-2 to promote dichlorvos induced apoptosis in HK-2 cells

Abstract

miRNAs are crucially involved in cellular responses to exotic chemical toxins. However, the role of miRNAs in organophosphates induced cytotoxicity is poorly understood. In present study, we investigated the role of miR-513a-5p in dichlorvos induced cytotoxicity in human kidney cell line HK-2. We found that dichlorvos increased intracellular ROS level, upregulated miR-513a-5p expression and induced apoptosis in HK-2 cells. Moreover, overexpression of miR-513a-5p promoted apoptosis of HK-2 cells with or without exposure to dichlorvos while anti-miR-513a-5p partially suppressed dichlorvos induced apoptosis. Luciferase assay showed that miR-513a-5p could directly bind to the 3'-untranslated regions of Bcl-2. Furthermore, miR-513a-5p decreased the level of Bcl-2 and promoted dichlorvos induced apoptosis in HK-2 cells through the Bcl-2/Bax-Caspase-3 pathway. Taken together, our findings indicate that miR-513a-5p promotes dichlorvos induced apoptosis by targeting Bcl-2.

Keywords: Apoptosis; Bcl-2; Cytotoxicity; Dichlorvos; miR-513a-5p.