Abstract
Staphylococcus aureus (S. aureus) causes a broad variety of diseases. The spread of multidrugresistant S. aureus highlights the need to develop new ways to combat S. aureus infections. Sortase A (SrtA) can anchor proteins containing LPXTG binding motifs to the bacteria surface and plays a key role in S. aureus infections, making it a promising antivirulence target. In the present study, we used aSrtA activity inhibition assay to discover that isovitexin, a Chinese herbal product, can inhibit SrtA activity with an IC50 of 28.98 μg/ml. Using a fibrinogenbinding assay and a biofilm formation assay, we indirectly proved the SrtA inhibitory activity of isovitexin. Additionally, isovitexin treatment decreased the amount of staphylococcal protein A (SpA) on the surface of the cells. These data suggest that isovitexin has the potential to be an anti-infective drug against S. aureus via the inhibition of sortase activity.
Keywords:
Staphylococcus aureu; antivirulence; inhibitor; isovitexin; sortase A.
MeSH terms
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Aminoacyltransferases / antagonists & inhibitors*
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Aminoacyltransferases / genetics
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Aminoacyltransferases / isolation & purification
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Aminoacyltransferases / metabolism
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Anti-Infective Agents / pharmacology*
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Apigenin / pharmacology*
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Bacterial Adhesion / drug effects
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Bacterial Proteins / antagonists & inhibitors*
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification
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Bacterial Proteins / metabolism
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Biofilms / drug effects*
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Biofilms / growth & development
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Cysteine Endopeptidases / genetics
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Cysteine Endopeptidases / isolation & purification
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Cysteine Endopeptidases / metabolism
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Drugs, Chinese Herbal / pharmacology
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Enzyme Inhibitors / pharmacology
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Inhibitory Concentration 50
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / enzymology
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Staphylococcus aureus / pathogenicity
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Staphylococcus aureus / physiology*
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Virulence Factors / metabolism
Substances
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Anti-Infective Agents
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Bacterial Proteins
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Drugs, Chinese Herbal
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Enzyme Inhibitors
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Virulence Factors
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Apigenin
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Aminoacyltransferases
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sortase A
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Cysteine Endopeptidases
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isovitexin