GSH and enzyme responsive nanospheres based on self-assembly of green tea polyphenols and BSA used for target cancer chemotherapy

Colloids Surf B Biointerfaces. 2019 Jan 1:173:654-661. doi: 10.1016/j.colsurfb.2018.10.037. Epub 2018 Oct 17.

Abstract

Developing safe and effective stimuli-responsive nanocarriers is very important for tumor chemotherapy. In this work, bovine serum albumin (BSA) and green tea polyphenol (TP) were used to prepare glutathione (GSH) and enzyme (trypsin) responsive nanocarriers for doxorubicin (DOX). These nanocarriers were further modified with folate, briefly named as DOX@BSA-TP-FA NSs. The diameter of nanocarriers was about 220 nm. The DOX loading efficiency and loading amount were 86.4% and 23.5 wt%, respectively. The cellular uptake, apoptosis, and GSH and trypsin responsive release properties of these nanocarriers were investigated.

Keywords: Anti-cancer; Control release; Drug delivery; Folic acid; Stimuli-responsive.

MeSH terms

  • Antibiotics, Antineoplastic / chemistry
  • Antibiotics, Antineoplastic / pharmacology*
  • Apoptosis / drug effects
  • Cell Survival / drug effects
  • Doxorubicin / chemistry
  • Doxorubicin / pharmacology*
  • Drug Carriers*
  • Drug Liberation
  • Folate Receptors, GPI-Anchored / metabolism
  • Folic Acid / chemistry
  • Folic Acid / metabolism
  • Glutathione / chemistry
  • Humans
  • Kinetics
  • MCF-7 Cells
  • Molecular Targeted Therapy
  • Nanospheres / chemistry*
  • Nanospheres / ultrastructure
  • Polyphenols / chemistry
  • Polyphenols / isolation & purification
  • Protein Binding
  • Serum Albumin, Bovine / chemistry*
  • Tea / chemistry
  • Trypsin / chemistry*

Substances

  • Antibiotics, Antineoplastic
  • Drug Carriers
  • Folate Receptors, GPI-Anchored
  • Polyphenols
  • Tea
  • Serum Albumin, Bovine
  • Doxorubicin
  • Folic Acid
  • Trypsin
  • Glutathione