[Substrate specificity of restriction endonuclease VspI]

S Kh Degtiarev; V E Repin; N I Rechkunova; V E Chizhikov; E G Malygin

[Substrate specificity of restriction endonuclease VspI]

Bioorg Khim. 1987 Mar;13(3):420-1.

[Article in Russian]

Authors

S Kh Degtiarev, V E Repin, N I Rechkunova, V E Chizhikov, E G Malygin

PMID: 3036169

Abstract

The recognition sequence and cleavage point of restriction endonuclease VspI have been determined as 5'-AT decreases TAAT. This enzyme is not isoschizomer of any known restriction endonucleases. DNA pBR322 contains a single VspI recognition sequence in position 3539. Therefore this enzyme may be used for cloning DNA in the VspI site in AmpR-gene of pBR322.

Publication types

English Abstract

MeSH terms

Base Sequence
DNA Restriction Enzymes / genetics*
DNA Restriction Enzymes / isolation & purification
Deoxyribonucleases, Type II Site-Specific*
Substrate Specificity
Vibrio / enzymology*

Substances

DNA Restriction Enzymes
endodeoxyribonuclease VspI
Deoxyribonucleases, Type II Site-Specific